Inheritance and expression of transgenes through anther culture of transgenic hot pepper.

نویسندگان

  • Young Soon Kim
  • Yong In Kuk
  • Kyung-Moon Kim
چکیده

Anther cultures have been developed from transgenic donor peppers carrying HPT/J1-1. Eight out of sixteen plants produced from an anther culture set pepper fruits. Southern blot analysis of donors revealed two independent plants with a single copy of the integrated transgene. PCR and RT-PCR results showed the inheritance of HPT/J1-1 and expression of J1-1 in A1. All A1 progeny derived from transgenic anthers had resistance to hygromycin. They grew normally and showed similar phenotypes to the wild-type. Therefore, the use of an anther culture system coupled with genetic transformation in breeding programs will greatly facilitate the genetic improvement of pepper plants.

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عنوان ژورنال:
  • Zeitschrift fur Naturforschung. C, Journal of biosciences

دوره 62 9-10  شماره 

صفحات  -

تاریخ انتشار 2007